Natalie Cappaert is an Assistant Professor at the Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands. Her thesis work was carried out in the Hearing Research Laboratories of the University of Utrecht. During her postdoctoral training her interest shifted toward the central nervous system. In her recent research on the network properties of the hippocampus and the entorhinal cortex, she has applied a combination of in vitro voltage sensitive dye imaging and extracellular recordings. In her VENI project (2004-2007) she studied theta oscillations and functional connectivity. In current project, the micro-circuitry of the perirhinal cortex is characterized, in particular to understand the functioning of the perirhinal gate, by investigating the relations between the participating excitatory and inhibitory neurons and their intercommunication.
To better understand the network properties, she also studies anatomical connectivity of the hippocampus and parahippocampal region, together with Prof. Menno Witter (Kavli Institute, Norwegian University of Science and Technology, Trondheim) and Niels van Strien (Kavli Institute) by developing an interactive connectome of the three-dimensional organization of the projection patterns between and within the hippocampal formation, the parahippocampal region and the retrosplenial cortex (Van Strien et al., 2009). The functional properties of this structural connectome were investigated with a graph analysis (Biniciewicz et al., 2016).
Natalie L.M. Cappaert, PhD
University of Amsterdam
Swammerdam Institute for Life Sciences
1098 XH Amsterdam, The Netherlands
Email: n.cappaert at(@) uva.nl
Personal website: http://www.uva.nl/contact/medewerkers/item/n.cappaert.html?f=cappaert
Distinct Spatiotemporal Activation Patterns of the Perirhinal-Entorhinal Network in Response to Cortical and Amygdala Input.
Distinct Spatiotemporal Activation Patterns of the Perirhinal-Entorhinal Network in Response to Cortical and Amygdala Input. Front Neural Circuits. 2016;10:44 Authors: Willems JG, Wadman WJ, Cappaert NL Abstract The perirhinal (PER) and entorhinal cortex (EC) receive input from the agranular insular cortex (AiP) and the subcortical lateral amygdala (LA) and the main output area is the hippocampus. Information transfer through the PER/EC network however, is not always guaranteed. It is hypothesized that this network actively regulates the (sub)cortical activity transfer to the hippocampal network and that the inhibitory system is involved in this function. This study determined the recruitment by the AiP and LA afferents in PER/EC network with the use of voltage sensitive dye (VSD) imaging in horizontal mouse brain slices. Electrical stimulation (500 μA) of the AiP induced activity that gradually propagated predominantly in the rostro-caudal direction: from the PER to the lateral EC (LEC). In the presence of 1 μM of the competitive γ-aminobutyric acid (GABAA) receptor antagonist bicuculline, AiP stimulation recruited the medial EC (MEC) as well. In contrast, LA stimulation (500 μA) only induced activity in the deep layers of the PER. In the presence of bicuculline, the initial population activity in the PER propagated further towards the superficial layers and the EC after a delay. The latency of evoked responses decreased with increasing stimulus intensities (50-500 μA) for both the AiP and LA stimuli. The stimulation threshold for evoking responses in the PER/EC network was higher for the LA than for the AiP. This study showed that the extent of the PER/EC network activation depends on release of inhibition. When GABAA dependent inhibition is reduced, both the AiP and the LA activate spatially overlapping regions, although in a distinct spatiotemporal fashion. It is therefore hypothesized that the inhibitory network regulates excitatory activity from both cortical and subcortical areas that has to be transmitted through the PER/EC network. PMID: 27378860 [PubMed - as supplied by publisher]