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Dr. NLM Cappaert

Natalie Cappaert is an Assistant Professor at the Swammerdam Institute for Life Sciences, University of Amsterdam, the Netherlands. Her thesis work was carried out in the Hearing Research Laboratories of the University of Utrecht. During her postdoctoral training her interest shifted toward the central nervous system. In her recent research on the network properties of the hippocampus and the entorhinal cortex, she has applied a combination of in vitro voltage sensitive dye imaging and extracellular recordings. In her VENI project (2004-2007) she studied theta oscillations and functional connectivity. In current project, the micro-circuitry of the perirhinal cortex is characterized, in particular to understand the functioning of the perirhinal gate, by investigating the relations between the participating excitatory and inhibitory neurons and their intercommunication.

To better understand the network properties, she also studies anatomical connectivity of the hippocampus and parahippocampal region, together with Prof. Menno Witter (Kavli Institute, Norwegian University of Science and Technology, Trondheim) and Niels van Strien (Kavli Institute) by developing an interactive connectome of the three-dimensional organization of the projection patterns between and within the hippocampal formation, the parahippocampal region and the retrosplenial cortex (Van Strien et al., 2009). The functional properties of this structural connectome were investigated with a graph analysis (Biniciewicz et al., 2016).

Contact Details

Natalie L.M. Cappaert, PhD
Assistant professor
University of Amsterdam
Swammerdam Institute for Life Sciences
Sciencepark 904
1098 XH Amsterdam, The Netherlands
Email: n.cappaert at(@) uva.nl

Personal website: http://www.uva.nl/contact/medewerkers/item/n.cappaert.html?f=cappaert

Meeting/Conference Poster/Abstract
Posters & Abstracts Click link to download (opens in new window).
SFN 2012 N.L.M. Cappaert, M.P. Witter, N.M. van Strien. 2012. New features in the 2012 release of the open source (para)hippocampal connectome of the rat. Poster at the Society for Neuroscience conference, New Orleans, USA.
SFN 2011 J. Sugar, M.P. Witter, N.M. van Strien, N. Cappaert. 2011. “The retrosplenial cortex: Intrinsic connectivity and connections with the (para)hippocampal region in the rat. An interactive connectome.” Poster at the Society for Neuroscience conference, Washington, USA.
FENS 2010 NLM Cappaert, TR Werkman, JC Baayen, MP Witter , R de Haan, WJ Wadman. 2010. "Evoked responses in hippocampal dentate gyrus tissue of epileptic patients: A voltage Sensitive dye imaging stud." Poster at the Federation of European Neurosciences conference, Amsterdam, The Netherlands.
SFN 2006 Natalie L.M. Cappaert & Wytse J. Wadman. 2006. “ Propagation and synchronization of theta oscillations the hippocampus and entorhinal cortex of the rat in vitro”. Poster at the Society for Neuroscience conference, USA.
SFN 2005 Natalie L.M. Cappaert, Wytse J. Wadman, Menno P. Witter. 2005. “Spatiotemporal analyses of interactions between entorhinal and CA1 projections to the subiculum of the rat”. Poster at the Society for Neuroscience conference, USA.

cappaert n[author]

cappaert n[author]: Latest results from PubMed
  • The perirhinal (PER) and lateral entorhinal (LEC) cortex function as a gateway for information transmission between (sub)cortical areas and the hippocampus. It is hypothesized that the amygdala, a key structure in emotion processing, can modulate PER-LEC neuronal activity before information enters the hippocampal memory pathway. This study determined the integration of synaptic activity evoked by simultaneous neocortical and amygdala electrical stimulation in PER-LEC deep layer principal neurons...

  • The perirhinal (PER) - lateral entorhinal (LEC) network plays a pivotal role in the information transfer between the neocortex and the hippocampus. Anatomical studies have shown that the connectivity is organized bi-directionally: the superficial layers consist of projections running from the neocortex via the PER-LEC network to the hippocampus while the deep layers form the output pathway back to the neocortex. Although these pathways are characterized anatomically, the functional organization...

  • The perirhinal (PER) and lateral entorhinal (LEC) cortex form an anatomical link between the neocortex and the hippocampus. However, neocortical activity is transmitted through the PER and LEC to the hippocampus with a low probability, suggesting the involvement of the inhibitory network. This study explored the role of interneuron mediated inhibition, activated by electrical stimulation in the agranular insular cortex (AiP), in the deep layers of the PER and LEC. Activated synaptic input by AiP...

  • The perirhinal (PER) and entorhinal cortex (EC) receive input from the agranular insular cortex (AiP) and the subcortical lateral amygdala (LA) and the main output area is the hippocampus. Information transfer through the PER/EC network however, is not always guaranteed. It is hypothesized that this network actively regulates the (sub)cortical activity transfer to the hippocampal network and that the inhibitory system is involved in this function. This study determined the recruitment by the AiP...

  • CONCLUSIONS: This study demonstrates that CBZ still reduced the activity in the DG, although the patients were clinically diagnosed as pharmacoresistant for CBZ. This suggests that in the human epileptic brain, the targets of CBZ, the voltage-gated Na(+) channels, are still sensitive to CBZ, although we used a relative high concentration and it is not possibility to assess the actual CBZ concentration that reached the target in the patient. We also concluded that the effect of CBZ was found in...

  • Graph theory was used to analyze the anatomical network of the rat hippocampal formation and the parahippocampal region (van Strien et al., Nat Rev Neurosci 10(4):272-282, 2009). For this analysis, the full network was decomposed along the three anatomical axes, resulting in three networks that describe the connectivity within the rostrocaudal, dorsoventral and laminar dimensions. The rostrocaudal network had a connection density of 12% and a path length of 2.4. The dorsoventral network had a...

  • Peripheral nerve injury leads to Wallerian degeneration, followed by regeneration, in which functionality and morphology of the nerve are restored. We previously described that deficiency for complement component C6, which prevents formation of the membrane attack complex, slows down degeneration and results in an earlier recovery of sensory function after sciatic nerve injury compared to WT animals. In this study, we determine whether C6(-/-) rats have an intrinsic trait that affects sciatic...

  • CONCLUSIONS: Our data suggests that the ex vivo electrophysiological method is complementary to the classical behavioral foot flick test in that it allows a detailed time analysis of the degeneration and early regeneration phases at a high spatial and temporal sensitivity. The results were well-matched with observations made with immunohistochemical and morphological methods.

  • Maternal exposure to the neurotoxin methylmercury (MeHg) has been shown to have adverse effects on neural development of the offspring in man. Little is known about the underlying mechanisms by which MeHg affects the developing brain. To explore the neurodevelopmental defects and the underlying mechanism associated with MeHg exposure, the cerebellum and cerebrum of Wistar rat pups were analyzed by [(18)F]FDG PET functional imaging, field potential analysis, and microarray gene expression...

  • Most deep brain stimulators apply rectangular monophasic voltage pulses. By modifying the stimulus shape, it is possible to optimize stimulus efficacy and find the best compromise between clinical effect, minimal side effects and power consumption of the stimulus generator. In this study, we compared the efficacy of three types of charge-balanced biphasic pulses (CBBPs, nominal duration 100 μs) in isolated sciatic nerves and in in vitro hippocampal brain slices of the rat. Using these two...

  • A connectome is an indispensable tool for brain researchers, since it quickly provides comprehensive knowledge of the brain's anatomical connections. Such knowledge lies at the basis of understanding network functions. Our first comprehensive and interactive account of brain connections comprised the rat hippocampal-parahippocampal network. We have now added all anatomical connections with the retrosplenial cortex (RSC) as well as the intrinsic connections of this region, because of the...

  • Theta oscillations (4-12 Hz) are associated with learning and memory and are found in the hippocampus and the entorhinal cortex (EC). The spatio-temporal organization of rhythmic activity in the hippocampal-EC complex was investigated in vitro. The voltage sensitive absorption dye NK3630 was used to record the changes in aggregated membrane voltage simultaneously from the neuronal networks involved. Oscillatory activity at 7.0 Hz (range, 5.8-8.2) was induced in the slice with the muscarinic...

  • Converging evidence suggests that each parahippocampal and hippocampal subregion contributes uniquely to the encoding, consolidation and retrieval of declarative memories, but their precise roles remain elusive. Current functional thinking does not fully incorporate the intricately connected networks that link these subregions, owing to their organizational complexity; however, such detailed anatomical knowledge is of pivotal importance for comprehending the unique functional contribution of...

  • The subiculum and the entorhinal cortex (EC) are important structures in processing and transmitting information between the neocortex and the hippocampus. The subiculum potentially receives information from the EC through two routes. In addition to a direct projection from EC to the subiculum, there is an indirect polysynaptic connection. The latter uses a number of possible pathways, which all converge onto the final projection from the hippocampal field CA1 to the subiculum. In this series of...

  • The antitumor agent cisplatin has dose-limiting side effects such as ototoxicity. Systemical co-treatment with anti-oxidants like 4-methylthiobenzoic acid (MTBA) and sodium thiosulfate (STS) provides protection against cisplatin ototoxicity. However, systemically administered protective agents may reduce the chemotherapeutic effect of cisplatin. Local application of the protective agents could avoid this undesirable effect. In the present study, we aimed at suppressing cisplatin-induced...

  • The effect of noise exposure on the auditory system is well known from animal studies. However, most of the studies concern short-term exposure conditions. The purpose of the present research was to find the dose-effect curve for hearing loss in rats following 5 days of noise exposure. Three groups of eight Wag/Rij rats were exposed to broad band noise at levels of 90, 100 and 110 dB SPL for 8 hours/day and 5 consecutive days. An additional group of eight rats served as the control group....

  • The present study was designed to compare the ototoxic effects of volatile ethyl benzene in guinea pigs and rats. Rats showed deteriorated auditory thresholds in the mid-frequency range, based on electrocochleography, after 550-ppm ethyl benzene (8 h/day, 5 days). Outer hair cell (OHC) loss was found in the corresponding cochlear regions. In contrast, guinea pigs showed no threshold shifts and no OHC loss after exposure to much higher ethyl benzene levels (2500 ppm, 6 h/day, 5 days)....

  • The effects on hearing of simultaneous exposure to the ototoxic organic solvent ethyl benzene and broad-band noise were evaluated in rats. The effects of three ethyl benzene concentrations (0, 300 or 400 ppm) and three noise levels (95 or 105 dB(lin) SPL or background noise at 65 dB(lin) SPL) and all their combinations were investigated for a 5 day exposure at 8 h/day. Distortion product otoacoustic emissions and compound action potentials were affected after 105 dB noise alone, and after 105 dB...

  • Rats were exposed to ethyl benzene at 0, 300, 400 and 550 ppm for 8 hours/day for 5 consecutive days. Three to six weeks after the exposure, auditory function was tested by measuring compound action potentials (CAP) in the frequency range of 1-24 kHz and 2f1-f2 distortion product otoacoustic emissions (DPOAEs) in the frequency range of 4-22.6 kHz. In addition, outer hair cell (OHC) loss was quantified by histological examination. The lowest concentration ethyl benzene had no effect on any of the...

  • Exposure to organic solvents has been shown to be ototoxic in animals and there is evidence that these solvents can induce hearing loss in humans. In this study, the effects of inhalation of the possibly ototoxic solvent ethyl benzene on the cochlear function and morphology were evaluated using three complementary techniques: (1) reflex modification audiometry (RMA), (2) electrocochleography and (3) histological examination of the cochleas. Rats were exposed to either ethyl benzene (800 ppm, 8...

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